Hi all I am wanting to go back through my male line of Fletchers ... I am not sure which test would be best for me as I would imagine the female will throw a spanner in the works any suggestions.
You have a choice of four types of genetic genealogy tests:
1.
Y-STR tests "short tandem repeats"("micro-satellites") on the Y chromosome and can identify or exclude a direct paternal lineage within genealogic time. Any intervening female ancestors will break the Y-inheritance chain. Depending on the commonness of the surname, Y-STR is more or less associated with surnames. You may find you have Y-STR matches with men named Harrison, which could suggest that this was the surname of your 2nd great-grandfather's biological father.
2.
Y-SNP tests single nucleotide polymorphisms on the Y chromosome and relates to paternal ancestry before genealogic time. Again, the Y chromosome does not pass through a female ancestor.
3.
mtDNA is analogous to SNP testing, but for mitochondria instead of the Y chromosome. Mitochondria are passed to both genders, but only by mothers; males do not pass on their mitochondria to their children.
4,
AuDNA ("au" meaning autosomal) tests genetic blocks in all chromosomes and can identify similar genetic inheritances up to a limit of about five generations. It is not limited as to ancestors' genders
To trace paternal lineage, I recommend a Y-STR test of at least 37 markers. It can look as far back as 24 generations, roughly about the time of universal surnames. More recent relationships (where both parties share a direct paternal ancestor) will also show as matches.
My opinion is that, at this time, Family Tree DNA is the provider of choice for these tests. Prices are reasonable (as compared to others), reliability and customer service are good, the database (for searching) is large, and support & tools are excellent. I also recommend joining a project for a surname or geographical area in order to obtain further support; this also argues for FTDNA.