No, Ralph's father was born in 1658, his father in 1620 and his father around 1600.
Ralph and Mary Trelfa moved to Backford around 1770 with all of their children, except Thomas.
And some of their descendants returned to Middlewich where most children were baptised, others then moved to Liverpool City centre.
I understand from a co- worker, that Mary Holland's parents were Daniel (1678) and Elizabeth Downes and his parents, Johann and Sara Cooke, though I have not seen documented evidence.
Can you say more about the DNA testing, it is potentially ground breaking in the hands of top class researchers.